2016 ; Vol.26-4: 684~692
|Author||Woori Kwak, Kwondo Kim, Chul Lee, Chanho Lee, Jungsun Kang, Kyungjin Cho, Sook Hee Yoon, Dae-Kyung Kang, Heebal Kim, Jaeyoung Heo, Seoae Cho|
|Place of duty||C&K Genomics, Seoul 08826, Republic of Korea,Interdisciplinary Program in Bioinformatics, Seoul National University, Seoul 08826, Republic of Korea|
|Title||Comparative Analysis of the Complete Genome of Lactobacillus plantarum GB-LP2 and Potential Candidate Genes for Host Immune System Enhancement|
J. Microbiol. Biotechnol.2016 ;
|Abstract||Acute respiratory virus infectious diseases are a growing health problem, particularly among
children and the elderly. Much effort has been made to develop probiotics that prevent
influenza virus infections by enhancing innate immunity in the respiratory tract until vaccines
are available. Lactobacillus plantarum GB-LP2, isolated from a traditional Korean fermented
vegetable, has exhibited preventive effects on influenza virus infection in mice. To identify the
molecular basis of this strain, we conducted a whole-genome assembly study. The single
circular DNA chromosome of 3,284,304 bp was completely assembled and 3,250 proteinencoding
genes were predicted. Evolutionarily accelerated genes related to the phenotypic
trait of anti-infective activities for influenza virus were identified. These genes encode three
integral membrane proteins, a teichoic acid export ATP-binding protein and a glucosamine -
fructose-6-phosphate aminotransferase involved in host innate immunity, the nonspecific
DNA-binding protein Dps, which protects bacteria from oxidative damage, and the response
regulator of the three-component quorum-sensing regulatory system, which is related to the
capacity of adhesion to the surface of the respiratory tract and competition with pathogens.
This is the first study to identify the genetic backgrounds of the antiviral activity in
L. plantarum strains. These findings provide insight into the anti-infective activities of
L. plantarum and the development of preventive probiotics.|
|Key_word||Lacatobacillus plantarum GB-LP2, comparative genomics, host immune enhancement, anti-viral effect|
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