2016 ; Vol.26-3: 579~587
|Author||Sukyung Kang, Jae Sung Lee, Hai Chon Lee, Michael C. Petriello, Bae Yong Kim, Jeong Tae Do, Dae-Seog Lim, Hong Gu Lee, Sung Gu Han|
|Place of duty||Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul 05029, Republic of Korea|
|Title||Phytoncide Extracted from Pinecone Decreases LPS-Induced Inflammatory Responses in Bovine Mammary Epithelial Cells|
J. Microbiol. Biotechnol.2016 ;
|Abstract||Mastitis is a prevalent inflammatory disease that remains one of the main causes of poor
quality of milk. Phytoncides are naturally occurring anti-inflammatory compounds derived
from plants and trees. To determine if treatment with phytoncide could decrease the severity
of lipopolysaccharide (LPS)-induced inflammatory responses, mammary alveolar epithelial
cells (MAC-T) were pretreated with phytoncide (0.02% and 0.04% (v/v)) followed by LPS
treatment (1 and 25 μg/ml). The results demonstrated that phytoncide downregulated LPSinduced
pro-inflammatory cyclooxygenase-2 (COX-2) expression. Additionally, LPS-induced
activation of ERK1/2, p38, and Akt was attenuated by phytoncide. Treatment of cells with
known pharmacological inhibitors of ERK1/2 (PD98059), p38 (SB203580), and Akt (LY294002)
confirmed the association of these signaling pathways with the observed alterations in COX-2
expression. Moreover, phytoncide attenuated LPS-induced NF-κB activation and superoxide
production, and, finally, treatment with phytoncide increased Nrf2 activation. Results suggest
that phytoncide can decrease LPS-induced inflammation in MAC-T cells.|
|Key_word||mastitis, lipopolysaccharide, MAC-T cell, inflammation, COX-2|
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