Journal of Microbiology and Biotechnology
The Korean Society for Microbiology and Biotechnology publishes the Journal of Microbiology and Biotechnology.

2016 ; Vol.26-6: 1011~1017

AuthorYoung Hwan Lee, Na-Kyoung Lee, Hyun-Dong Paik
Place of dutyDepartment of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul 05029, Republic of Korea
TitleAntimicrobial Characterization of Inula britannica against Helicobacter pylori on Gastric Condition
PublicationInfo J. Microbiol. Biotechnol.2016 ; Vol.26-6
AbstractThe antimicrobial effects of methanol and ethanol extracts of Inula britannica against several Helicobacter pylori strains (26695, J99, and SS1) were evaluated in vitro, to determine their applicability as functional foods. In the paper disc diffusion method, the antimicrobial effects of the I. britannica extracts against the H. pylori strains were apparent. Viable cell counting also showed that the extracts at 100 μg/ml concentration dramatically decreased the viability of the H. pylori strains. In particular, the methanol and ethanol extracts at a concentration of 100 μg/ml reduced the H. pylori SS1 cell number to 2.46 log CFU/ml and 1.08 log CFU/ml, respectively. In the presence of 100 μg/ml extracts, the urease production of H. pylori SS1 was decreased to more than 30%, whereas that of H. pylori J99 and H. pylori 26695 was decreased to about 20%, relative to the controls. The extracts inhibited the attachment of the H. pylori strains to human gastric AGS cells as well as caused the detachment of already attached H. pylori cells. In addition, the H. pylori morphology was changed to a coccoidal shape in the presence of the extracts. In conclusion, the I. britannica extracts were effective against H. pylori strains in vitro, irrespective of genotype status, and could therefore be used as novel functional foods.
Full-Text
Key_wordInula britannica, Helicobacter pylori, antimicrobial effect, urease, morphology
References
  1. Bisignano C, Filocamo A, La Camera E, Zummo S, Fera MT, Mandalari G. 2013. Antibacterial activities of almond skins on cagA-positive and -negative clinical isolates of Helicobacter pylori. BMC Microbiol. 13: 103.
    Pubmed CrossRef Pubmed Central
  2. Bonifácio BV, Santos Ramos MA, Sil va PB, Bauab TM. 2014. Antimicrobial activity of natural products against Helicobacter pylori: a review. Ann. Clin. Microbiol. Antimicrob. 13: 54.
    CrossRef
  3. Burger O, Ofek I, Tabak M, Weiss E, Sharon N, Neeman I. 2000. A high molecular weight constituent of cranberry juice inhibits Helicobacter pylori adhesion to human gastric mucus. FEMS Immunol. Med. Microbiol. 22: 1-7.
  4. Chitapanarux T, Thonsawat S, Pisespongsa P, Leerapun A, Kijdamrongthum P. 2015. Effect of Bifidobacterium longum on PPI-based triple therapy for eradication of Helicobacter pylori:a randomized, double-blind placebo-controlled study. J. Funct. Foods 7: 390-397.
  5. Covacci A, Telford JL, Del Giudice G, Parsonnet J, Rappuoli R. 1999. Helicobacter pylori virulence and genetic geography. Science 284: 1328-1333.
    Pubmed CrossRef
  6. Digu C, Cornea CP, Ioni L, Brîndu e E, Farca N, Bobit D, Matei F. 2014. Studies on antimicrobial activity of Inula helenium L Romanian cultivar. Rom. Biotechnol. Lett. 19: 96999704.
  7. Hamasaki N, Ishii E, Tominaga K, Tezuka Y, Nagaoka T, Kadota S, et al. 2000. Highly selective antibacterial activity of novel alkyl quinolone alkaloids from a Chinese herbal medicine, Gosyuyu (Wu-Chu-Yu), against Helicobacter pylori in vivo. Microbiol. Immunol. 44: 9-15.
    Pubmed CrossRef
  8. Jeong JY, Berg DE. 2000. Mouse-colonizing Helicobacter pylori SS1 is unusually susceptible to metronidazole due to two complementary reductase activities. Antimicrob. Agents Chemother. 44: 3127-3132.
    Pubmed CrossRef Pubmed Central
  9. Joo JS, Park KC, Song JY, Kim DH, Lee KJ, Kwon YC, et al. 2010. A thin-layer liquid cul ture technique for the growth of Helicobacter pylori. Helicobacter 15: 295-302.
    Pubmed CrossRef
  10. Khan AL, Hussain J, Hamayun M, Gilani SA, Ahmad S, Rehman G, et al. 2010. Secondary metabolites from Inula britannica L. and their biological activities. Molecules 15:1562-1577.
    Pubmed CrossRef
  11. Kusters JG, Gerrits MM, Van Strijp JAG, VandenbrouckeGrauls CMJE. 1997. Coccoid forms of Helicobacter pylori are the morphologic manifestation of cell death. Infect. Immun. 65: 3672-3679.
    Pubmed Pubmed Central
  12. Lee JH, Dinh VN, Ma JY, Kim YB, Kim SK, Paik HD. 2010. Screening of antiviral medicinal plants against avian influenza virus H1N1 for food safety. Korean J. Food Sci. Anim. Resour. 30: 345-350.
    CrossRef
  13. Lee NK, Lee JH, Lee YJ, Ahn SH, Eom SJ, Paik HD. 2013. Antimicrobial effect of Inula britannica flower extract against methicillin-resistant Staphylococcus aureus. Korean J. Microbiol. Biotechnol. 63: 550-560.
    CrossRef
  14. Lee NK, Lee EK, Paik HD. 2013. Potential probiotic properties of phytase-producing Lactobacillus salivarius FC113. Ann. Microbiol. 41: 335-340.
    CrossRef
  15. Lee Y, Chang HC. 2008. Isolation and characterization of kimchi lactic acid bacteria showing anti-Helicobacter pylori activity. Korean J. Microbiol. Biotechnol. 36: 106-114.
  16. Lesbors-Pantoflickova D, Corthésy-Theulaz I, Blum AL. 2007. Helicobacter pylori and probiotics. J. Nutr. 137: 812S-817S.
  17. Martini S, D’Addario C, Colacevin A, Focardi S, Borghini F, Santucci A, et al. 2009. Antimicrobial activity against Helicobacter pylori strains and antioxidant properties of blackberry leaves (Rubus ulmifolius) and isolated compounds. Int. J. Antimicrob. Agents 34: 50-59.
    Pubmed CrossRef
  18. Malekzadeh F, Ehsanifar H, Shahamat M, Levin M, Colwell RR. 2001. Antibacterial activity of black myrobalan (Terminalia chebulaetz) against Helicobacter pylori. Int. J. Antimicrob. Agents 18: 85-88.
    CrossRef
  19. Matsushima M, Suzuki T, Masui A, Mine T, Takagi A. 2013. Cranberry extract suppresses interleukin-8 secretion from stomach cells stimulated by Helicobacter pylori in every clinically separated strain but inhibits growth in part of the strains. J. Funct. Foods 5: 729-735.
    CrossRef
  20. Meine GC, Rota C, Dietz J, Sekine S, Prolla JC. 2011. Relationship between cagA-positive Helicobacter pylori infection and risk of gastric cancer: a case control study in Porto Alegro, RS, Brazil. Arq. Gastroenterol. 48: 41-45.
    Pubmed
  21. Moon SH, Lee JH, Kim KT, Park YS, Nah SY, Ahn DU, Paik HD. 2013. Antimicrobial effect of 7-O-butylnaringenin, a novel flavonoid and various natural flavonoids against Helicobacter pylori strains. Int. J. Environ. Res. Public. Health. 10: 5459-5469.
    Pubmed CrossRef Pubmed Central
  22. Nilius M, Strohle A, Bode G, Malfertheiner P. 1993. Coccoid-like forms of Helicobacter pylori. Enzyme activity and antigenicity. Zentralbl. Bakteriol. 280: 259-272.
    CrossRef
  23. Oleastro M, Ménard A. 2013. The role of Helicobacter pylori outer membrane proteins in adherence and pathogenesis. Biology 2: 1110-1134.
    Pubmed CrossRef Pubmed Central
  24. Razmara RS, Daneshfar A, Sahraei R. 2010. Solubility of quercetin in water + methanol and water + ethanol from (292.8 to 333.8) K. J. Chem. Eng. Data 55: 3934-3936.
    CrossRef
  25. Stingel K, Reuse HD. 2005. Stayling alive overdosed: how does Helicobacter pylori control urease activity? Int. J. Med. Microbiol. 295: 307-315.
    Pubmed CrossRef
  26. Worku ML, Sidebotham RL, Walker MM, Keshavarz T, Karim QN. 1999. The relationship between Helicobacter pylori motility, morphology and phase of growth: implications for gastric colonization and pathology. Microbiology 145: 2803-2811.
    Pubmed CrossRef
  27. Zhang XL, Jiang AM, Ma ZY, Li XB, Xiong YY, Dou JF, Wang JF. 2015. The synthetic antimicrobial peptide pexigana and its nanoparticles (PNPs) exhibit the anti-Helicobacter pylori activity in vitro and in vivo. Molecules 20: 3972-3985.
    Pubmed CrossRef



Copyright © 2009 by the Korean Society for Microbiology and Biotechnology.
All right reserved. Mail to jmb@jmb.or.kr
Online ISSN: 1738-8872    Print ISSN: 1017-7825    Powered by INFOrang Co., Ltd