2016 ; Vol.26-6: 1011~1017
|Author||Young Hwan Lee, Na-Kyoung Lee, Hyun-Dong Paik|
|Place of duty||Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul 05029, Republic of Korea|
|Title||Antimicrobial Characterization of Inula britannica against Helicobacter pylori on Gastric Condition|
J. Microbiol. Biotechnol.2016 ;
|Abstract||The antimicrobial effects of methanol and ethanol extracts of Inula britannica against several
Helicobacter pylori strains (26695, J99, and SS1) were evaluated in vitro, to determine their
applicability as functional foods. In the paper disc diffusion method, the antimicrobial effects
of the I. britannica extracts against the H. pylori strains were apparent. Viable cell counting also
showed that the extracts at 100 μg/ml concentration dramatically decreased the viability of the
H. pylori strains. In particular, the methanol and ethanol extracts at a concentration of 100 μg/ml
reduced the H. pylori SS1 cell number to 2.46 log CFU/ml and 1.08 log CFU/ml, respectively.
In the presence of 100 μg/ml extracts, the urease production of H. pylori SS1 was decreased to
more than 30%, whereas that of H. pylori J99 and H. pylori 26695 was decreased to about 20%,
relative to the controls. The extracts inhibited the attachment of the H. pylori strains to human
gastric AGS cells as well as caused the detachment of already attached H. pylori cells. In
addition, the H. pylori morphology was changed to a coccoidal shape in the presence of the
extracts. In conclusion, the I. britannica extracts were effective against H. pylori strains in vitro,
irrespective of genotype status, and could therefore be used as novel functional foods.|
|Key_word||Inula britannica, Helicobacter pylori, antimicrobial effect, urease, morphology|
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