2016 ; Vol.26-1: 151~159
|Author||Munazza Fatima, Najam-us-Sahar Sadaf Zaidi, Deeba Amraiz, Farhan Afzal|
|Place of duty||Atta-ur-Rahman School of Applied Biosciences (ASAB), National University of Sciences and Technology (NUST), H-12, Kashmir Highway, Islamabad, Pakistan|
|Title||In Vitro Antiviral Activity of Cinnamomum cassia and Its Nanoparticles Against H7N3 Influenza A Virus|
J. Microbiol. Biotechnol.2016 ;
|Abstract||Nanoparticles have wide-scale applications in various areas, including medicine, chemistry,
electronics, and energy generation. Several physical, biological, and chemical methods have
been used for synthesis of silver nanoparticles. Green synthesis of silver nanoparticles using
plants provide advantages over other methods as it is easy, efficient, and eco-friendly.
Nanoparticles have been extensively studied as potential antimicrobials to target pathogenic
and multidrug-resistant microorganisms. Their applications recently extended to development
of antivirals to inhibit viral infections. In this study, we synthesized silver nanoparticles using
Cinnamomum cassia (Cinnamon) and evaluated their activity against highly pathogenic avian
influenza virus subtype H7N3. The synthesized nanoparticles were characterized using UVVis
absorption spectroscopy, scanning electron microscopy, and Fourier transform infrared
spectroscopy. Cinnamon bark extract and its nanoparticles were tested against H7N3
influenza A virus in Vero cells and the viability of cells was determined by tetrazolium dye
(MTT) assay. The silver nanoparticles derived from Cinnamon extract enhanced the antiviral
activity and were found to be effective in both treatments, when incubated with the virus prior
to infection and introduced to cells after infection. In order to establish the safety profile,
Cinnamon and its corresponding nanoparticles were tested for their cytotoxic effects in Vero
cells. The tested concentrations of extract and nanoparticles (up to 500 μg/ml) were found
non-toxic to Vero cells. The biosynthesized nanoparticles may, hence, be a promising
approach to provide treatment against influenza virus infections.|
|Key_word||Antiviral activity, Nanoparticles, H7N3, Influenza, Cinnamomum cassia, Cinnamon bark|
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