2015 ; Vol.25-12: 2043~2048
|Author||Seung-Hak Cho, Suk-Ho Kang, Yea-Eun Lee, Sung-Jo Kim, Young-Bin Yoo, Yeong-Seok Bak, Jung-Beom Kim|
|Place of duty||Division of Enteric Diseases, Center for Infectious Diseases, Korea National Institute of Health, Osong 363-951, Republic of Korea|
|Title||Distribution of Toxin Genes and Enterotoxins in Bacillus thuringiensis Isolated from Microbial Insecticide Products|
J. Microbiol. Biotechnol.2015 ;
|Abstract||Bacillus thuringiensis microbial insecticide products have been applied worldwide. Although a
few cases of B. thuringiensis foodborne illness have been reported, little is known about the
toxigenic properties of B. thuringiensis isolates. The aims of this study were to estimate the
pathogenic potential of B. thuringiensis selected from microbial insecticide products, based on
its possession of toxin genes and production of enterotoxins. Fifty-two B. thuringiensis strains
selected from four kinds of microbial insecticide products were analyzed. PCR assay for
detection of toxin genes and immunoassay for detection of enterotoxins were performed. The
hemolysin BL complex as a major enterotoxin was produced by 17 (32.7%), whereas the nonhemolytic
enterotoxin complex was detected in 1 (1.9%) of 52 B. thuringiensis strains. However,
cytK, entFM, and ces genes were not detected in any of the tested B. thuringiensis strains. The
potential risk of food poisoning by B. thuringiensis along with concerns over B. thuringiensis
microbial insecticide products has gained attention recently. Thus, microbial insecticide
products based on B. thuringiensis should be carefully controlled.|
|Key_word||Bacillus thuringiensis, microbial insecticide, enterotoxin, toxin gene|
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