2015 ; Vol.25-9: 1460~1466
|Author||Hyun-jung Park, Jang Won Yoon, Eun-Jeong Heo, Eun-Kyoung Ko, Ki-Yeon Kim, Young-Jo Kim, Hyang-Jin Yoon, Sung-Hwan Wee, Yong Ho Park, Jin San Moon|
|Place of duty||Ministry of Food and Drug Safety, Cheongwon 363-951, Republic of Korea|
|Title||Antibiotic Resistance and Virulence Potentials of Shiga Toxin-Producing Escherichia coli Isolates from Raw Meats of Slaughterhouses and Retail Markets in Korea|
J. Microbiol. Biotechnol.2015 ;
|Abstract||In this study, the prevalence of Shiga toxin-producing Escherichia coli (STEC) was investigated
among raw meat or meat products from slaughterhouses and retail markets in South Korea,
and their potential for antibiotic resistance and virulence was further analyzed. A total of 912
raw meats, including beef, pork, and chicken, were collected from 2008 to 2009. E. coli strains
were frequently isolated in chicken meats (176/233, 75.9%), beef (102/217, 42.3%), and pork
(109/235, 39.2%). Putative STEC isolates were further categorized, based on the presence or
absence of the Shiga toxin (stx) genes, followed by standard O-serotyping. Polymerase chain
reaction assays were used to detect the previously defined virulence genes in STEC, including
Shiga toxins 1 and Shiga toxin 2 (stx1 and 2), enterohemolysin (ehxA), intimin (eaeA), STEC
autoagglutination adhesion (saa), and subtilase cytotoxin (subAB). All carried both stx1 and eae
genes, but none of them had the stx2, saa, or subAB genes. Six (50.0%) STEC isolates possessed
the ehxA gene, which is known to be encoded by the 60-megadalton virulence plasmid. Our
antibiogram profiling demonstrated that some STEC strains, particularly pork and chicken
isolates, displayed a multiple drug-resistance phenotype. RPLA analysis revealed that all the
stx1-positive STEC isolates produced Stx1 only at the undetectable level. Altogether, these
results imply that the locus of enterocyte and effacement (LEE)-positive strains STEC are
predominant among raw meats or meat products from slaughterhouses or retail markets in
|Key_word||Retail meat, Shiga toxin, Stx-producing E. coli, Antibiogram, polymerase chain reaction|
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