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 2015 ; Vol.25-8: 1246~1256
| Author | Yanqun Hu, Lili Chen, Chuan Wang, Yafeng Xie, Zhixi Chen, Liangzhuan Liu, Zehong Su, Yimou Wu |
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| Place of duty | Department of Microbiology and Immunology, University of South China, Hengyang, Hunan 421001, P.R. China |
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| Title | Transcriptional Analysis of 10 Selected Genes in a Model of Penicillin G Induced Persistence of Chlamydophila psittaci in HeLa Cells |
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| PublicationInfo |
J. Microbiol. Biotechnol.2015 ;
Vol.25-8
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| Abstract | Chlamydophila psittaci is an important intracellular pathogen. Persistent infection is an
important state of the host-parasite interaction in this chlamydial infection, which plays a
significant role in spreading the organism within animal populations and in causing chronic
chlamydiosis and serious sequelae. In this study, a C. psittaci persistent infection cell model
was induced by penicillin G, and real-time quantitative PCR was used to study the
transcriptional levels of 10 C. psittaci genes (dnaA, dnaK, ftsW, ftsY, grpE, rpsD, incC, omcB,
CPSIT_0846, and CPSIT_0042) in acute and penicillin-G-induced persistent infection cultures.
Compared with the acute cultures, the penicillin-G-treated cultures showed a reduced
chlamydial inclusion size and a significantly decreased number of elementary body particles.
Additionally, some enlarged aberrant reticulate body particles were present in the penicillin-
G-treated cultures but not the acute ones. The expression levels of genes encoding products for
cell division (FtsW, FtsY) and outer membrane protein E encoding gene (CPSIT_0042) were
downregulated (p < 0.05) from 6 h post-infection onward in the persistent infection cultures.
Also from 6 h post-infection, the expression levels of DnaA, DnaK, IncC, RpsD, GrpE, and
CPSIT_0846 were upregulated (p < 0.05); however, the expression level of OmcB in the
persistent infection was almost the same as that in the acute infection (p > 0.05). These results
provide new insight regarding molecular activities that accompany persistence of C. psittaci,
which may play important roles in the pathogenesis of C. psittaci infection. |
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| Key_word | Chlamydophila psittaci, persistent infection, transcription, penicillin G |
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| References |
Abdelrahman YM, Belland RJ. 2005. The chlamydial developmental cycle. FEMS Microbiol. Rev. 29: 949-959. Beatty WL, Byrne GI, Morrison RP. 1993. Morphologic and antigenic characterization of interferon gamma-mediated persistent Chlamydia trachomatis infection in vitro. Proc. Natl. Acad. Sci. USA 90: 3998-4002. Beatty WL, Morrison RP, Byrne GI. 1994. Persistent chlamydiae: from cell culture to a paradigm for chlamydial pathogenesis. Microbiol. Rev. 58: 686-699. Beatty WL, Morrison RP, Byrne GI. 1995. Reactivation of persistent Chlamydia trachomatis infection in cell culture. Infect. Immun. 63: 199-205. Belland RJ, Zhong G, Crane DD, Hogan D, Sturdevant D, Sharma J, et al. 2003. Genomic transcriptional profiling of the developmental cycle of Chlamydia trachomatis. Proc. Natl. Acad. Sci. USA 100: 8478-8483. Branley JM, Roy B, Dwyer DE, Sorrell TC. 2008. Real-time PCR detection and quantitation of Chlamydophila psittaci in human and avian specimens from a veterinary clinic cluster. Eur. J. Clin. Microbiol. Infect. Dis. 27: 269-273. Byrne GI, Ouellette SP, Wang Z, Rao JP, Lu L, Beatty WL, Hudson AP. 2001. Chlamydia pneumoniae expresses genes required for DNA replication but not cytokinesis during persistent infection of HEp-2 cells. Infect. Immun. 69: 5423-5429. Cha SB, Yoo A, Park HT, Sung KY, Shin MK, Yoo HS. 2013. Analysis of transcriptional profiles to discover biomarker candidates in Mycobacterium avium subsp. paratuberculosisinfected macrophages, RAW 264.7. J. Microbiol. Biotechnol. 23: 1167-1175. Charles PG, Whitby M, Fuller AJ, Stirling R, Wright AA, Korman TM, et al. 2008. The etiology of community-acquired pneumonia in Australia: why penicillin plus doxycycline or a macrolide is the most appropriate therapy. Clin. Infect. Dis. 46: 1513-1521. Clark RB, Schatzki PF, Dalton HP. 1982. Ultrastructural analysis of the effects of erythromycin on the morphology and developmental cycle of Chlamydia trachomatis HAR-13. Arch. Microbiol. 133: 278-282. Coles AM, Reynolds DJ, Harper A, Devitt A, Pearce JH. 1993. Low-nutrient induction of abnormal chlamydial development: a novel component of chlamydial pathogenesis? FEMS Microbiol. Lett. 106: 193-200. Dielissen PW, Teunissen DA, Lagro-Janssen AL. 2013. Chlamydia p revalence in t he g eneral p opulation: is t here a sex difference? A systematic review. BMC Infect. Dis. 13: 534. Donachie WD. 1993. The cell cycle of Escherichia coli. Annu. Rev. Microbiol. 47: 199-230. Fadel S, Eley A. 2007. Chlamydia trachomatis OmcB protein is a surface-exposed glycosaminoglycan-dependent adhesin. J. Med. Microbiol. 56: 15-22. Fadel S, Eley A. 2008. Differential glycosaminoglycan binding of Chlamydia trachomatis OmcB protein from serovars E and LGV. J. Med. Microbiol. 57: 1058-1061. Gaede W, Reckling KF, Dresenkamp B, Kenklies S, Schubert E, Noack U, et al. 2008. Chlamydophila psittaci infections in humans during an outbreak of psittacosis from poultry in Germany. Zoonoses Public Health 55: 184-188. Gerard HC, Freise J, Wang Z, Roberts G, Rudy D, KraussOpatz B, et al. 2002. Chlamydia trachomatis genes whose products are related to energy metabolism are expressed differentially in active vs. persistent infection. Microbes Infect. 4: 13-22. Gerard HC, Whittum-Hudson JA, Schumacher HR, Hudson AP. 2006. Synovial Chlamydia trachomatis up regulates expression of a panel of genes similar to that transcribed by Mycobacterium tuberculosis during persistent infection. Ann. Rheum. Dis. 65: 321-327. Goellner S, Schubert E, Liebler-Tenorio E, Hotzel H, Saluz HP, Sachse K. 2006. Transcriptional response patterns of Chlamydophila psittaci in different in vitro models of persistent infection. Infect. Immun. 74: 4801-4808. Gupta R, Salhan S, Mittal A. 2009. Seroprevalence of antibodies against Chlamydia trachomatis inclusion membrane proteins B and C in infected symptomatis women. J. Infect. Dev. Ctries. 3: 191-198. Gupta R, Srivastava P, Vardhan H, Salhan S, Mittal A. 2009. Host immune responses to chlamydial inclusion membrane proteins B and C in Chlamydia trachomatis infected women with or without fertility disorders. Reprod. Biol. Endocrinol. 7: 38. Gupta R, Vardhan H, Srivastava P, Salhan S, Mittal A. 2009. Modulation of cytokines and transcription factors (T-Bet and GATA3) in CD4 enriched cervical cells of Chlamydia trachomatis infected fertile and infertile women upon stimulation with chlamydial inclusion membrane proteins B and C. Reprod. Biol. Endocrinol. 7: 84. Harkinezhad T, Verminnen K, De Buyzere M, Rietzschel E, Bekaert S, Vanrompay D. 2009. Prevalence of Chlamydophila psittaci infections in a human population in contact with domestic and companion birds. J. Med. Microbiol. 58: 1207-1212. Hogan RJ, Mathews SA, Mukhopadhyay S, Summersgill JT, Timms P. 2004. Chlamydial persistence: beyond the biphasic paradigm. Infect. Immun. 72: 1843-1855. Hughes C, Maharg P, Rosario P, Herrell M, Bratt D, Salgado J, Howard D. 1997. Possible nosocomial transmission of psittacosis. Infect. Control Hosp. Epidemiol. 18: 165-168. Jha HC, Srivastava P, Vardhan H, Singh LC, Bhengraj AR, Prasad J, Mittal A. 2011. Chlamydia pneumoniae heat shock protein 60 is associated with apoptotic signaling pathway in human atheromatous plaques of coronary artery disease patients. J. Cardiol. 58: 216-225. Kaleta EF, Taday EM. 2003. Avian host range of Chlamydophila spp. based on isolation, antigen detection and serology. Avian Pathol. 32: 435-461. Kim JK. 2013. Epidemiological trends of sexually transmitted infections among women in Cheonan, South Korea, 20062012. J. Microbiol. Biotechnol. 23: 1484-1490. Kokab A, Jennings R, Eley A, Pacey AA, Cross NA. 2010. Analysis of modulated gene expression in a model of interferongammainduced persistence of Chlamydia trachomatis in HEp-2 cells. Microb. Pathog. 49: 217-225. Lambden PR, Pickett MA, Clarke IN. 2006. The effect of penicillin on Chlamydia trachomatis DNA replication. Microbiology 152: 2573-2578. Lee MJ, Min BJ, Choung HK, Kim N, Kim YA, Khwarg SI. 2014. Genome-wide DNA methylation profiles according to Chlamydophila psittaci infection and the response to doxycycline treatment in ocular adnexal lymphoma. Mol. Vis. 20: 1037-1047. Mathews S, George C, Flegg C, Stenzel D, Timms P. 2001. Differential expression of ompA, ompB, pyk, nlpD and Cpn0585 genes between normal and interferon-gamma treated cultures of Chlamydia pneumoniae. Microb. Pathog. 30: 337-345. Matsumoto A, Manire GP. 1970. Electron microscopic observations on the effects of penicillin on the morphology of Chlamydia psittaci. J. Bacteriol. 101: 278-285. Maurer AP, Mehlitz A, Mollenkopf HJ, Meyer TF. 2007. Gene expression profiles of Chlamydophila pneumoniae during the developmental cycle and iron depletion-mediated persistence. PLoS Pathog. 3: e83. Mehta SJ, Miller RD, Ramirez JA, Summersgill JT. 1998. Inhibition of Chlamydia pneumoniae replication in HEp-2 cells by interferon-gamma: role of tryptophan catabolism. J. Infect. Dis. 177: 1326-1331. Ouellette SP, Hatch TP, Abdel Rahman YM, Rose LA, Belland RJ, Byrne GI. 2006. Global transcriptional upregulation in the absence of increased translation in Chlamydia during IFNgamma-mediated host cell tryptophan starvation. Mol. Microbiol. 62: 1387-1401. Peters J, Hess S, Endlich K, Thalmann J, Holzberg D, Kracht M, et al. 2005. Silencing or permanent activation: host-cell responses in models of persistent Chlamydia pneumoniae infection. Cell. Microbiol. 7: 1099-1108. Pettengill MA, Marques-da-Silva C, Avila ML, d’Arc dos Santos Oliveira S, Lam VW, Ollawa I, et al. 2012. Reversible inhibition of Chlamydia trachomatis infection in epithelial cells due to stimulation of P2X4 receptors. Infect. Immun. 80: 4232-4238. Raulston JE. 1997. Response of Chlamydia trachomatis serovar E to iron restriction in vitro and evidence for iron-regulated chlamydial proteins. Infect. Immun. 65: 4539-4547. Schoborg RV. 2011. Chlamydia persistence - a tool to dissect chlamydia-host interactions. Microbes Infect. 13: 649-662. Shen L, Li M, Zhang YX. 2004. Chlamydia trachomatis sigma28 recognizes the fliC promoter of Escherichia coli and responds to heat shock in chlamydiae. Microbiology 150: 205-215. Smith K A, B radley KK, S tobierski M G, T eng elsen LA, National Association of State Public Health Veterinarians Psittacosis Compendium C. 2005. Compendium of measures to control Chlamydophila psittaci (formerly Chlamydia psittaci) infection among humans (psittacosis) and pet birds, 2005. J. Am. Vet. Med. Assoc. 226: 532-539. Theegarten D, Anhenn O, Hotzel H, Wagner M, Marra A, Stamatis G, et al. 2004. A comparative ultrastructural and molecular biological study on Chlamydia psittaci infection in alpha-1 antitrypsin deficiency and non-alpha-1 antitrypsin deficiency emphysema versus lung tissue of patients with hamartochondroma. BMC Infect. Dis. 4: 38. Thies FL, Karch H, Hartung HP, Giegerich G. 1999. Cloning and expression of the dnaK gene of Campylobacter jejuni and antigenicity of heat shock protein 70. Infect. Immun. 67:1194-1200. Wolf K, Fischer E, Hackstadt T. 2000. Ultrastructural analysis of developmental events in Chlamydia pneumoniaeinfected cells. Infect. Immun. 68: 2379-2385. Wyrick PB. 2010. Chlamydia trachomatis persistence in vitro:an overview. J. Infect. Dis. 201(Suppl 2): S88-S95. Zhong G. 2011. Chlamydia trachomatis secretion of proteases for manipulating host signaling pathways. Front. Microbiol. 2: 14.
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