2015 ; Vol.25-7: 1136~1145
|Author||Gangping Li, Min Yang, Kan Zhou, Lei Zhang, Lugao Tian, Shangze Lv, Yu Jin, Wei Qian, Hanhua Xiong, Rong Lin, Yu Fu, Xiaohua Hou|
|Place of duty||Division of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, P.R. China|
|Title||Diversity of Duodenal and Rectal Microbiota in Biopsy Tissues and Luminal Contents in Healthy Volunteers|
J. Microbiol. Biotechnol.2015 ;
|Abstract||The diverse microbial communities that colonize distinct segments of the gastrointestinal tract
are intimately related to aspects of physiology and the pathology of human health. However,
most recent studies have focused on the rectal or fecal microbiota, and the microbial signature
of the duodenum is poorly studied. In this study, we compared the microbiota in duodenal
and rectal samples to illustrate the characteristic microbial signatures of the duodenum in
healthy adults. Nine healthy volunteers donated biopsies and luminal contents from the
duodenum and rectum. To determine the composition and diversity of the microbiota, 454-
pyrosequencing of bacterial 16S rRNA was performed and multiple bioinformatics analyses
were applied. The α-diversity and phylogenetic diversity of the microbiota in the duodenal
samples were higher than those of the rectal samples. There was higher biodiversity among
the microbiota isolated from rectal biopsies than feces. Proteobacteria were more highly
represented in the duodenum than in the rectum, both in the biopsies and in the luminal
contents from the healthy volunteers (38.7% versus 12.5%, 33.2% versus 5.0%, respectively).
Acinetobacter and Prevotella were dominant in the duodenum, whereas Bacteroides and
Prevotella were dominant in the rectum. Additionally, the percentage of OTUs shared in biopsy
groups was far higher than in the luminal group (43.0% versus 26.8%) and a greater number of
genera was shared among the biopsies than the luminal contents. Duodenal samples
demonstrated greater biological diversity and possessed a unique microbial signature
compared with the rectum. The mucosa-associated microbiota was more relatively conserved
than luminal samples.|
|Key_word||Microbiota, duodenum, rectum, biopsy, mucus, feces|
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