2015 ; Vol.25-8: 1195~1204
|Author||Fang Wang, Ting Yu, Guohong Huang, Da Cai, Xiaolin Liang, Haiyan Su, Zhenjun Zhu, Danlei Li, Yang Yang, Peihong Shen, Ruifeng Mao, Lian Yu, Mouming Zhao, Quanyang Li|
|Place of duty||College of Light Industry and Food Engineering, Guangxi University, Nanning 530004, P.R. China|
|Title||Gut Microbiota Community and Its Assembly Associated with Age and Diet in Chinese Centenarians|
J. Microbiol. Biotechnol.2015 ;
|Abstract||Increasing evidence suggests that gut microbiota underpin the development of health and
longevity. However, our understanding of what influences the composition of this community
of the longevous has not been adequately described. Therefore, illumina sequencing analysis
was performed on the gut microbiota of centenarians (aged 100-108 years; RC) and younger
elderlies (aged 85-99 years; RE) living in Bama County, Guangxi, China and the elderlies (aged
80-92 years; CE) living in Nanning City, Guangxi, China. In addition, their diet was monitored
using a semiquantitative dietary questionary (FFQ 23). The results revealed the abundance of
Roseburia and Escherichia was significantly greater, whereas that of Lactobacillus,
Faecalibacterium, Parabacteroides, Butyricimonas, Coprococcus, Megamonas, Mitsuokella, Sutterella,
and Akkermansia was significantly less in centenarians at the genus level. Both clustering
analysis and UniFraq distance analysis showed structural segregation with age and diet
among the three populations. Using partial least square discriminate analysis and redundancy
analysis, we identified 33 and 34 operational taxonomic units (OTUs) as key OTUs that were
significantly associated with age and diet, respectively. Age-related OTUs were characterized
as Ruminococcaceae, Clostridiaceae, and Lachnospiraceae, and the former two were increased in
the centenarians; diet-related OTUs were classified as Bacteroidales, Lachnospiraceae, and
Ruminococcaceae. The former two were deceased, whereas the later one was increased, in the
high-fiber diet. The age and high-fiber diet were concomitant with changes in the gut
microbiota of centenarians, suggesting that age and high-fiber diet can establish a new
structurally balanced architecture of gut microbiota that may benefit the health of
|Key_word||centenarians, gut microbiota, illumina sequencing, age, diet|
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