2014 ; Vol.24-12: 1622~1628
|Author||Juyi Park, Soon-Kwang Hong, Yong Keun Chang|
|Place of duty||Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Republic of Korea|
|Title||Production of DagA, a β-Agarase, by Streptomyces lividans in Glucose Medium or Mixed-Sugar Medium Simulating Microalgae Hydrolysate|
J. Microbiol. Biotechnol.2014 ;
|Abstract||DagA, a β-agarase, was produced by cultivating a recombinant Streptomyces lividans in a
glucose medium or a mixed-sugar medium simulating microalgae hydrolysate. The optimum
composition of the glucose medium was identified as 25 g/l glucose, 10 g/l yeast extract, and
5 g/l MgCl2·6H2O. With this, a DagA activity of 7.26 U/ml could be obtained. When a mixedsugar
medium containing 25 g/l of sugars was used, a DagA activity of 4.81 U/ml was
obtained with very low substrate utilization efficiency owing to the catabolic repression of
glucose against the other sugars. When glucose and galactose were removed from the
medium, an unexpectedly high DagA activity of about 8.7 U/ml was obtained, even though a
smaller amount of sugars was used. It is recommended for better substrate utilization and
process economics that glucose and galactose be eliminated from the medium, by being
consumed by some other useful applications, before the production of DagA.|
|Key_word||DagA, Streptomyces lividans, mixed-sugar medium, microalgae hydrolysate|
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