2014 ; Vol.24-5: 667~674
|Author||Youri Jang, Younghoon Lim, Keun Kim|
|Place of duty||Department of Bioscience and Biotechnology, The University of Suwon, Gyeonggi-do 445-743, Republic of Korea|
|Title||Saccharomyces cerevisiae Strain Improvement Using Selection, Mutation, and Adaptation for the Resistance to Lignocellulose-Derived Fermentation Inhibitor for Ethanol Production|
J. Microbiol. Biotechnol.2014 ;
|Abstract||Twenty-five Saccharomyces cerevisiae strains were screened for the highest sugar tolerance,
ethanol-tolerance, ethanol production, and inhibitor resistance, and S. cerevisiae KL5 was
selected as the best strain. Inhibitor cocktail (100%) was composed of 75 mM formic acid,
75 mM acetic acid, 30 mM furfural, 30 mM hydroxymethyl furfural (HMF), and 2.7 mM
vanillin. The cells of strain KL5 were treated with γ-irradiation, and among the survivals, KL5-
G2 with improved inhibitor resistance and the highest ethanol yield in the presence of
inhibitor cocktail was selected. The KL5-G2 strain was adapted to inhibitor cocktail by
sequential transfer of cultures to a minimal YNB medium containing increasing concentrations
of inhibitor cocktail. After 10 times of adaptation, most of the isolated colonies could grow in
YNB with 80% inhibitor cocktail, whereas the parental KL5 strain could not grow at all.
Among the various adapted strains, the best strain (KL5-G2-A9) producing the highest ethanol
yield in the presence of inhibitor cocktail was selected. In a complex YP medium containing
60% inhibitor cocktail and 5% glucose, the theoretical yield and productivity (at 48 h) of KL5-
G2-A9 were 81.3% and 0.304 g/l/h, respectively, whereas those of KL5 were 20.8% and 0.072
g/l/h, respectively. KL5-G2-A9 reduced the concentrations of HMF, furfural, and vanillin in
the medium in much faster rates than KL5.|
|Key_word||Lignocellulose-derived fermentation inhibitor, resistance, adaptation, Saccharomyces cerevisiae, ethanol production|
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