2014 ; Vol.24-3: 363~370
|Author||Yuanqing Hu, Jinlin Huang, Qiuchun Li, Yuwei Shang, Fangzhe Ren, Yang Jiao, Zhicheng Liu, Zhiming Pan, Xin-an Jiao|
|Place of duty||Jiangsu Key Laboratory of Zoonosis, Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Jiangsu 225009, PR China|
|Title||Use of In Vivo-Induced Antigen Technology to Identify In Vivo-Expressed Genes of Campylobacter jejuni During Human Infection|
J. Microbiol. Biotechnol.2014 ;
|Abstract||Campylobacter jejuni is a prevalent foodborne pathogen worldwide. Human infection by C.
jejuni primarily arises from contaminated poultry meats. Genes expressed in vivo may play an
important role in the pathogenicity of C. jejuni. We applied an immunoscreening method, in
vivo-induced antigen technology (IVIAT), to identify in vivo-induced genes during human
infection by C. jejuni. An inducible expression library of genomic proteins was constructed
from sequenced C. jejuni NCTC 11168 and was then screened using adsorbed, pooled human
sera obtained from clinical patients. We successfully identified 24 unique genes expressed in
vivo. These genes were implicated in metabolism, molecular biosynthesis, genetic information
processing, transport, and other processes. We selected six genes with different functions to
compare their expression levels in vivo and in vitro using real-time RT-PCR. The results
showed that the selected six genes were significantly upregulated in vivo but not in vitro. In
short, these identified in vivo-induced genes may contribute to human infection of C. jejuni,
some of which may be meaningful vaccine candidate antigens or diagnosis serologic markers
for campylobacteriosis. IVIAT may present a significant and efficient method for
understanding the pathogenicity mechanism of Campylobacter and for finding targets for its
prevention and control.|
|Key_word||Campylobacter jejuni, in vivo-induced antigen technology (IVIAT), in vivo-induced gene, foodborne pathogen|
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