Journal of Microbiology and Biotechnology
The Korean Society for Microbiology and Biotechnology publishes the Journal of Microbiology and Biotechnology.

2014 ; Vol.24-3: 363~370

AuthorYuanqing Hu, Jinlin Huang, Qiuchun Li, Yuwei Shang, Fangzhe Ren, Yang Jiao, Zhicheng Liu, Zhiming Pan, Xin-an Jiao
Place of dutyJiangsu Key Laboratory of Zoonosis, Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Jiangsu 225009, PR China
TitleUse of In Vivo-Induced Antigen Technology to Identify In Vivo-Expressed Genes of Campylobacter jejuni During Human Infection
PublicationInfo J. Microbiol. Biotechnol.2014 ; Vol.24-3
AbstractCampylobacter jejuni is a prevalent foodborne pathogen worldwide. Human infection by C. jejuni primarily arises from contaminated poultry meats. Genes expressed in vivo may play an important role in the pathogenicity of C. jejuni. We applied an immunoscreening method, in vivo-induced antigen technology (IVIAT), to identify in vivo-induced genes during human infection by C. jejuni. An inducible expression library of genomic proteins was constructed from sequenced C. jejuni NCTC 11168 and was then screened using adsorbed, pooled human sera obtained from clinical patients. We successfully identified 24 unique genes expressed in vivo. These genes were implicated in metabolism, molecular biosynthesis, genetic information processing, transport, and other processes. We selected six genes with different functions to compare their expression levels in vivo and in vitro using real-time RT-PCR. The results showed that the selected six genes were significantly upregulated in vivo but not in vitro. In short, these identified in vivo-induced genes may contribute to human infection of C. jejuni, some of which may be meaningful vaccine candidate antigens or diagnosis serologic markers for campylobacteriosis. IVIAT may present a significant and efficient method for understanding the pathogenicity mechanism of Campylobacter and for finding targets for its prevention and control.
Full-Text
Key_wordCampylobacter jejuni, in vivo-induced antigen technology (IVIAT), in vivo-induced gene, foodborne pathogen
References
  1. Balaban M, Joslin SN, Hendrixson DR. 2009. FlhF and its GTPase activity are required for distinct processes in flagellar gene regulation and biosynthesis in Campylobacter jejuni. J. Bacteriol. 191: 6602-6611.
    CrossRef
  2. Cordwell SJ, Len AC, Touma RG, Scott NE, Falconer L, Jones D, et al. 2008. Identification of membrane-associated proteins from Campylobacter jejuni strains using complementary proteomics technologies. Proteomics 8: 122-139.
    CrossRef
  3. Dasti JI, Tareen AM, Lugert R, Zautner AE, Gross U. 2010. Campylobacter jejuni: a brief overview on pathogenicityassociated factors and disease-mediating mechanisms. Int. J. Med. Microbiol. 300: 205-211.
    CrossRef
  4. Gaasbeek EJ, van der Wal FJ, van Putten JP, de Boer P, van der Graaf-van Bloois L, de Boer AG, et al. 2009. Functional characterization of excision repair and RecA-dependent recombinational DNA repair in Campylobacter jejuni. J. Bacteriol. 191: 3785-3793.
    CrossRef
  5. Gu H, Zhu H, Lu C. 2009. Use of in vivo-induced antigen technology (IVIAT) for the identification of Streptococcus suis serotype 2 in vivo-induced bacterial protein antigens. BMC Microbiol. 9: 201.
    CrossRef
  6. Hang L, John M, Asaduzzaman M, Bridges EA, Vanderspurt C, Kirn TJ, et al. 2003. Use of in vivo-induced antigen technology (IVIAT) to identify genes uniquely expressed during human infection with Vibrio cholerae. Proc. Natl. Acad. Sci. USA 100: 8508-8513.
    CrossRef
  7. Hermans D, Pasmans F, Messens W, Martel A, Van Immerseel F, Rasschaert G, et al. 2012. Poultry as a host for the zoonotic pathogen Campylobacter jejuni. Vector Borne Zoonot. Dis. 12: 89-98.
    CrossRef
  8. Hofreuter D, Novik V, Galan JE. 2008. Metabolic diversity in Campylobacter jejuni enhances specific tissue colonization. Cell Host Microbe 4: 425-433.
    CrossRef
  9. Hu Y, Cong Y, Li S, Rao X, Wang G, Hu F. 2009. Identification of in vivo induced protein antigens of Salmonella enterica serovar Typhi during human infection. Sci. China C Life Sci. 52: 942-948.
    CrossRef
  10. Hu Y, Shang Y, Huang J, Wang Y, Ren F, Jiao Y, et al. 2013. A novel immunoproteomics method for identifying in vivoinduced Campylobacter jejuni antigens using pre-adsorbed sera from infected patients. BBA Gen. Subjects 1830: 52295235.
    CrossRef
  11. Jagusztyn-Krynicka EK, Laniewski P, Wyszynska A. 2009. Update on Campylobacter jejuni vaccine development for preventing human campylobacteriosis. Expert. Rev. Vaccines 8: 625-645.
    CrossRef
  12. Kaakoush NO, Raftery M, Mendz GL. 2008. Molecular responses of Campylobacter jejuni to cadmium stress. FEBS J. 275: 5021-5033.
    CrossRef
  13. Lowry JE, Goodridge L, Vernati G, Fluegel AM, Edwards WH, Andrews GP. 2010. Identification of Brucella abortus genes in elk (Cervus elaphus) using in vivo-induced antigen technology (IVIAT) reveals novel markers of infection. Vet. Microbiol. 142: 367-372.
    CrossRef
  14. M alik-Kale P, P arker C T, K onkel M E. 2 008. C ulture of Campylobacter jejuni with sodium deoxycholate induces virulence gene expression. J. Bacteriol. 190: 2286-2297.
    CrossRef
  15. Newell DG. 2001. Animal models of Campylobacter jejuni colonization and disease and the lessons to be learned from similar Helicobacter pylori models. J. Appl. Microbiol. 90: 57S67S.
  16. Pappas MG, Hajkowski R, Hockmeyer WT. 1983. Dot enzyme-linked immunosorbent assay (Dot-ELISA): a micro technique for the rapid diagnosis of visceral leishmaniasis. J. Immunol. Methods 64: 205-214.
    CrossRef
  17. Parkhill J, Wren BW, Mungall K, Ketley JM, Churcher C, Basham D, et al. 2000. The genome sequence of the foodborne pathogen Campylobacter jejuni reveals hypervariable sequences. Nature 403: 665-668.
    CrossRef
  18. Rathbun KM, Thompson SA. 2009. Mutation of PEB4 alters the outer membrane protein profile of Campylobacter jejuni. FEMS Microbiol. Lett. 300: 188-194.
    CrossRef
  19. Riedel CT, Cohn MT, Stabler RA, Wren B, Brøndsted L. 2012. Cellular response of Campylobacter jejuni to trisodium phosphate. Appl. Environ. Microbiol. 78: 1411-1415.
    CrossRef
  20. Rollins SM, Peppercorn A, Hang L, Hillman JD, Calderwood SB, Handfield M, Ryan ET. 2005. In vivo induced antigen technology (IVIAT). Cell Microbiol. 7: 1-9.
    CrossRef
  21. Trost M, Wehmhöner D, Kärst U, Dieterich G, Wehland J, Jänsch L. 2005. Comparative proteome analysis of secretory proteins from pathogenic and nonpathogenic Listeria species. Proteomics 5: 1544-1557.
    CrossRef
  22. Vigil PD, Alteri CJ, Mobley HLT. 2011. Identification of in vivo-induced antigens including an RTX family exoprotein required for uropathogenic Escherichia coli virulence. Infect. Immun. 79: 2335-2344.
    CrossRef
  23. Wiesner RS, Hendrixson DR, DiRita VJ. 2003. Natural transformation of Campylobacter jejuni requires components of a type II secretion system. J. Bacteriol. 185: 5408-5418.
    CrossRef
  24. Woodall CA, Jones MA, Barrow PA, Hinds J, Marsden GL, Kelly DL, et al. 2005. Campylobacter jejuni gene expression in the chick cecum: evidence for adaptation to a low-oxygen environment. Infect. Immun. 73: 5278-5285.
    CrossRef
  25. Young KT, Davis LM, Dirita VJ. 2007. Campylobacter jejuni:molecular biology and pathogenesis. Nat. Rev. Microbiol. 5:665-679.
    CrossRef
  26. Zautner AE, Herrmann S, Corso J, Tareen AM, Alter T, Groß U. 2011. Epidemiological association of different Campylobacter jejuni groups with metabolism-associated genetic markers. Appl. Environ. Microbiol. 77: 2359-2365.
    CrossRef
  27. Zilbauer M, Dorrell N, Wren BW, Bajaj-Elliott M. 2008. Campylobacter jejuni-mediated disease pathogenesis: an update. Trans. R. Soc. Trop. Med. Hyg. 102: 123-129.
    CrossRef
  28. Zou YX, Mo ZL, Hao B, Ye XH, Guo DS, Zhang PJ. 2010. Screening of genes expressed in vivo after infection by Vibrio anguillarum M3. Lett. Appl. Microbiol. 51: 564-569.
    CrossRef



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