2014 ; Vol.24-8: 1015~1025
|Author||Mehboob Ahmed, Lucas J. Stal, Shahida Hasnain|
|Place of duty||Department of Microbiology and Molecular Genetics, University of the Punjab, Lahore-54590, Pakistan,Department of Marine Microbiology, Royal Netherlands Institute for Sea Research (NIOZ), NL-4400 AC Yerseke, The Netherlands|
|Title||Biofilm Formation and Indole-3-Acetic Acid Production by Two Rhizospheric Unicellular Cyanobacteria|
J. Microbiol. Biotechnol.2014 ;
|Abstract||Microorganisms that live in the rhizosphere play a pivotal role in the functioning and
maintenance of soil ecosystems. The study of rhizospheric cyanobacteria has been hampered
by the difficulty to culture and maintain them in the laboratory. The present work investigated
the production of the plant hormone indole-3-acetic acid (IAA) and the potential of biofilm
formation on the rhizoplane of pea plants by two cyanobacterial strains, isolated from rice
rhizosphere. The unicellular cyanobacteria Chroococcidiopsis sp. MMG-5 and Synechocystis sp.
MMG-8 that were isolated from a rice rhizosphere, were investigated. Production of IAA by
Chroococcidiopsis sp. MMG-5 and Synechocystis sp. MMG-8 was measured under experimental
conditions (pH and light). The bioactivity of the cyanobacterial auxin was demonstrated
through the alteration of the rooting pattern of Pisum sativum seedlings. The increase in the
concentration of L-tryptophan and the time that this amino acid was present in the medium
resulted in a significant enhancement of the synthesis of IAA (r > 0.900 at p = 0.01). There was
also a significant correlation between the concentration of IAA in the supernatant of the
cyanobacteria cultures and the root length and number of the pea seedlings. Observations
made by confocal laser scanning microscopy revealed the presence of cyanobacteria on the
surface of the roots and also provided evidence for the penetration of the cyanobacteria in the
endorhizosphere. We show that the synthesis of IAA by Chroococcidiopsis sp. MMG-5 and
Synechocystis sp. MMG-8 occurs under different environmental conditions and that the auxin
is important for the development of the seedling roots and for establishing an intimate
symbiosis between cyanobacteria and host plants.|
|Key_word||Auxin, Bioassay, Cyanobacterial biofilms, Chroococcidiopsis sp, Synechocystis sp, Indole 3-Acetic Acid|
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