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Microbial Ecology and Diversity
Diversity and Saline Resistance of Endophytic Fungi Associated with Pinus thunbergii in Coastal Shelterbelts of Korea
1School of Biological Sciences, Seoul National University, Seoul 151-747, Republic of Korea, 2Warm-Temperate and Subtropical Forest Research Center, KFRI, Seogwipo 697-050, Republic of Korea
Received: October 14, 2013; Accepted: December 4, 2013
J. Microbiol. Biotechnol. 2014; 24(3): 324-333
Published March 28, 2014 https://doi.org/10.4014/jmb.1310.10041
Copyright © The Korean Society for Microbiology and Biotechnology.
Abstract
The Black Pine, Pinus thunbergii, is widely distributed along the eastern coast of Korea and its
importance as a shelterbelt was highlighted after tsunamis in Indonesia and Japan. The root
endophytic diversity of P. thunbergii was investigated in three coastal regions; Goseong, Uljin,
and Busan. Fungi were isolated from the root tips, and growth rates of pure cultures were
measured and compared between PDA with and without 3% NaCl to determine their saline
resistance. A total of 259 isolates were divided into 136 morphotypes, of which internal
transcribed spacer region sequences identified 58 species. Representatives of each major fungi
phylum were present: 44 Ascomycota, 8 Zygomycota, and 6 Basidiomycota. Eighteen species
exhibited saline resistance, many of which were Penicillium and Trichoderma species. Shoreline
habitats harbored higher saline-tolerant endophytic diversity compared with inland sites. This
investigation indicates that endophytes of P. thunbergii living closer to the coast may have
higher resistance to salinity and potentially have specific relationships with P. thunbergii.
Keywords
Coastal pine forest, Endophyte community, Salinity tolerance, Shelterbelt
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