Journal of Microbiology and Biotechnology
The Korean Society for Microbiology and Biotechnology publishes the Journal of Microbiology and Biotechnology.

2014 ; Vol.24-3: 371~378

AuthorLuca Dominici, Milena Villarini, Francesca Trotta, Ermanno Federici, Giovanni Cenci, Massimo Moretti
Place of dutyDepartment of Pharmaceutical Sciences, University of Perugia, Via del Giochetto, 06122 Perugia, Italy,Department of Chemistry, Biology and Biotechnology, University of Perugia, Via del Giochetto, 06122 Perugia, Italy
TitleProtective Effects of Probiotic Lactobacillus rhamnosus IMC501 in Mice Treated with PhIP
PublicationInfo J. Microbiol. Biotechnol.2014 ; Vol.24-3
AbstractThe aim of the present study was to investigate the antigenotoxic properties of the probiotic Lactobacillus rhamnosus IMC501; DNA damage was induced by one representative food mutagen, 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP). Mice were treated orally with suspensions of lactobacilli for 10 days before administration of food mutagen. During the treatment, the abundance of lactobacilli in feces, as assessed by qPCR analysis, increased, whereas β-glucuronidase and N-acetyl-β-glucosaminidase activities decreased. The extent of DNA damage was measured in colon and liver cells by comet assay. In colonocytes, diet supplementation with IMC501 resulted in a significant inhibition of DNA damage induced by PhIP. The results obtained in this in vitro study suggest that Lactobacillus rhamnosus IMC501 used as a dietary supplement can provide a useful integration of antimutagen food components of the normal diet, which are generally lower than the protective level.
Full-Text
Key_wordProbiotics, Lactobacillus rhamnosus, antigenotoxicity, comet assay, PhIP
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