2014 ; Vol.24-3: 371~378
|Author||Luca Dominici, Milena Villarini, Francesca Trotta, Ermanno Federici, Giovanni Cenci, Massimo Moretti|
|Place of duty||Department of Pharmaceutical Sciences, University of Perugia, Via del Giochetto, 06122 Perugia, Italy,Department of Chemistry, Biology and Biotechnology, University of Perugia, Via del Giochetto, 06122 Perugia, Italy|
|Title||Protective Effects of Probiotic Lactobacillus rhamnosus IMC501 in Mice Treated with PhIP|
J. Microbiol. Biotechnol.2014 ;
|Abstract||The aim of the present study was to investigate the antigenotoxic properties of the probiotic
Lactobacillus rhamnosus IMC501; DNA damage was induced by one representative food
mutagen, 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP). Mice were treated orally
with suspensions of lactobacilli for 10 days before administration of food mutagen. During the
treatment, the abundance of lactobacilli in feces, as assessed by qPCR analysis, increased,
whereas β-glucuronidase and N-acetyl-β-glucosaminidase activities decreased. The extent of
DNA damage was measured in colon and liver cells by comet assay. In colonocytes, diet
supplementation with IMC501 resulted in a significant inhibition of DNA damage induced by
PhIP. The results obtained in this in vitro study suggest that Lactobacillus rhamnosus IMC501
used as a dietary supplement can provide a useful integration of antimutagen food
components of the normal diet, which are generally lower than the protective level.|
|Key_word||Probiotics, Lactobacillus rhamnosus, antigenotoxicity, comet assay, PhIP|
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