2014 ; Vol.24-3: 313~323
|Author||Yuanyuan Li, Longqian Chen, Hongyu Wen, Tianjian Zhou, Ting Zhang, Xiali Gao|
|Place of duty||Jiangsu Key Laboratory for Resources and Environment Information Engineering, School of Environment and Spatial Informatics, China University of Mining and Technology, Xuzhou, 221116, China|
|Title||454 Pyrosequencing Analysis of Bacterial Diversity Revealed by a Comparative Study of Soils from Mining Subsidence and Reclamation Areas|
J. Microbiol. Biotechnol.2014 ;
|Abstract||Significant alteration in the microbial community can occur across reclamation areas suffering
subsidence from mining. A reclamation site undergoing fertilization practices and an adjacent
coal-excavated subsidence site (sites A and B, respectively) were examined to characterize the
bacterial diversity using 454 high-throughput 16S rDNA sequencing. The dominant taxonomic
groups in both the sites were Proteobacteria, Acidobacteria, Bacteroidetes, Betaproteobacteria,
Actinobacteria, Gammaproteobacteria, Alphaproteobacteria, Deltaproteobacteria, Chloroflexi,
and Firmicutes. However, the bacterial communities’ abundance, diversity, and composition
differed significantly between the sites. Site A presented higher bacterial diversity and more
complex community structures than site B. The majority of sequences related to Proteobacteria,
Gemmatimonadetes, Chloroflexi, Nitrospirae, Firmicutes, Betaproteobacteria, Deltaproteobacteria,
and Anaerolineae were from site A; whereas those related to Actinobacteria, Planctomycetes,
Bacteroidetes, Verrucomicrobia, Gammaproteobacteria, Nitriliruptoria, Alphaproteobacteria,
and Phycisphaerae originated from site B. The distribution of some bacterial groups and
subgroups in the two sites correlated with soil properties and vegetation due to reclamation
practice. Site A exhibited enriched bacterial community, soil organic matter (SOM), and total
nitrogen (TN), suggesting the presence of relatively diverse microorganisms. SOM and TN were
important factors shaping the underlying microbial communities. Furthermore, the specific
plant functional group (legumes) was also an important factor influencing soil microbial
community composition. Thus, the effectiveness of 454 pyrosequencing in analyzing soil
bacterial diversity was validated and an association between land ecological system restoration,
mostly mediated by microbial communities, and an improvement in soil properties in coalmining
reclamation areas was suggested.|
|Key_word||454 Pyrosequencing, bacterial diversity, mining soil reclamation, soil organic matter, total nitrogen|
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