2013 ; Vol.23-12: 1747~1756
|Author||Alba Beas-Catena, Asterio Sánchez-Mirón, Francisco Garía-Camacho, Antonio Contreras-Gómez, Emilio Molina-Grima|
|Place of duty||Department of Chemical Engineering, University of Almería, 04120 Almería, Spain|
|Title||The Effect of Spent Medium Recycle on Cell Proliferation, Metabolism and Baculovirus Production by the Lepidopteran Se301 Cell Line Infected at Very Low MOI|
J. Microbiol. Biotechnol.2013 ;
|Abstract||The aim of this paper was to study the effect of spent medium recycle on Spodoptera exigua
Se301 cell line proliferation, metabolism, and baculovirus production when grown in batch
suspension cultures in Ex-Cell 420 serum-free medium. The results showed that the recycle of
20% of spent medium from a culture in mid-exponential growth phase improved growth
relative to a control culture grown in fresh medium. Although both glucose and glutamine
were still present at the end of the growth phase, glutamate was always completely exhausted.
The pattern of the specific glucose and lactate consumption and production rates, as well as
the specific glutamine and glutamate consumption rates, suggests a metabolic shift at spent
medium recycle values of over 60%, with a decrease in the efficiency of glucose utilization and
an increase in glutamate consumption to fuel energy metabolism. Baculovirus infection
provoked a change in the metabolic pattern of Se301 cells, although a beneficial effect of spent
medium recycle was also observed. Both growth rate and maximum viable cell density
decreased relative to uninfected cultures. The efficiency of glucose utilization was
dramatically reduced in those cultures containing the lowest percentages of spent medium,
whereas glutamine and glutamate consumption was modulated, thereby suggesting that
infected cells were devoted to virus replication, retaining their ability to incorporate the
nutrients required to support viral replication. Recycle of 20% of spent medium increased
baculovirus production by around 90%, thus showing the link between cell growth and
|Key_word||Se301 cell line, baculovirus, spent medium recycle, metabolism, growth rate|
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