Journal of Microbiology and Biotechnology
The Korean Society for Microbiology and Biotechnology publishes the Journal of Microbiology and Biotechnology.

2013 ; Vol.23-12: 1757~1764

AuthorHuiju Gao, Xiang Chu, Yanwen Wang, Fei Zhou, Kai Zhao, Zhimei Mu, Qingxin Liu
Place of dutyCollege of Forestry, Shandong Agricultural University, Shandong 271018, China
TitleMedia Optimization for Laccase Production by Trichoderma harzianum ZF-2 Using Response Surface Methodology
PublicationInfo J. Microbiol. Biotechnol.2013 ; Vol.23-12
AbstractTrichoderma harzianum ZF-2 producing laccase was isolated from decaying samples from Shandong, China, and showed dye decolorization activities. The objective of this study was to optimize its culture conditions using a statistical analysis of its laccase production. The interactions between different fermentation parameters for laccase production were characterized using a Plackett-Burman design and the response surface methodology. The different media components were initially optimized using the conventional one-factor-at-atime method and an orthogonal test design, and a Plackett-Burman experiment was then performed to evaluate the effects on laccase production. Wheat straw powder, soybean meal, and CuSO4 were all found to have a significant influence on laccase production, and the optimal concentrations of these three factors were then sequentially investigated using the response surface methodology with a central composite design. The resulting optimal medium components for laccase production were determined as follows: wheat straw powder 7.63 g/l, soybean meal 23.07 g/l, (NH4)2SO4 1 g/l, CuSO4 0.51 g/l, Tween-20 1 g/l, MgSO4 1 g/l, and KH2PO4 0.6 g/l. Using this optimized fermentation method, the yield of laccase was increased 59.68 times to 67.258 U/ml compared with the laccase production with an unoptimized medium. This is the first report on the statistical optimization of laccase production by Trichoderma harzianum ZF-2.
Full-Text
Key_wordTrichoderma harzianum ZF-2, media optimization, laccase production, response surface methodology
References
  1. Abadulla E, Tzanov T, Costa S, Robra KH, Cavaco PA, Gubitz G. 2000. Decolorization and detoxication of textile dyes with a laccase from Trametes hirsuta. Appl. Environ. Microbiol. 66: 3357-3362.
    Pubmed CrossRef
  2. Assavanig A, Amornkitticharoen B, Ekpaisal N, Meevootisom V, Flegel TM. 1992. Isolation, characterization and function of laccase from Trichoderma. Appl. Microbiol. Biotechnol. 38:198-202.
    CrossRef
  3. Bollag JM, Leonowicz A. 1984. Comparative studies of extracellular fungal laccases. Appl. Environ. Microbiol. 48:849-854.
    Pubmed
  4. Box GEP, Wilson KB. 1951. On the experimental attainment of optimum conditions. J. Roy. Stat. Soc. B 13: 145.
  5. Chen T, Barton SC, Binyamin G, Gao Z, Zhang Y, Kim HH, et al. 2001. A miniature biofuel cell. J. Am. Chem. Soc. 123:8630-8631.
    Pubmed CrossRef
  6. Collins PJ, Dobson ADW. 1997. Regulation of laccase gene transcription in Trametes versicolor. Appl. Environ. Microbiol. 63: 3444-3450.
    Pubmed
  7. Dittmer JK, Patel NJ, Dhawale SW, Dhawale SS. 1997. Production of multiple laccase isoforms by Phanerochaete chrysosporium grown under nutrient sufficiency. FEMS Microbiol. Lett. 149: 65-70.
    CrossRef
  8. Eggert C, Temp U, Eriksson KEL. 1996. The ligninolytic system of the white-rot fungus Pycnoporus cinnabarinus: purification and characterization of the laccase. Appl. Environ. Microbiol. 62: 1151-1158.
    Pubmed
  9. Gao HJ, Wang YW, Zhang WT, Wang WL, Mu ZM. 2011. Isolation, identification and application in lignin degradation of an ascomycete GHJ-4. Afr. J. Biotechnol. 10: 4166-4174.
  10. Gianfreda L, Xu F, Bollag JM. 1999. Laccases: a useful group of oxidoreductive enzymes. Bioremediat. J. 3: 1-26.
    CrossRef
  11. Gochev VK, Krastanov AI. 2007. Isolation of laccase producing Trichoderma spp. Bulg. J. Agric. Sci. 13: 171-176.
  12. Gomes SA, Rebelo MJ. 2003. A new laccase biosensor for polyphenols determination. Sensors 3: 166-175.
    CrossRef
  13. Holker U, Dohse J, Hofer M. 2002. Extracellular laccases in ascomycetes Trichoderma atroviride and Trichoderma harzianum. Folia Microbiol. 47: 423-427.
    CrossRef
  14. Jing DB, Li PJ, Stagnitti F, Xiong XZ. 2007. Optimization of laccase production from Trametes versicolor by solid fermentation. Can. J. Microbiol. 53: 245-251.
    Pubmed CrossRef
  15. Kantelinen A, Hatakka A, Viikari L. 1989. Production of lignin peroxidase and laccase by Phlebia radiata. Appl. Microbiol. Biotechnol. 31: 234-239.
    CrossRef
  16. Lee IY, Jung KH, Lee CH, Park YH. 1999. Enhanced production of laccase in Trametes versicolor by the addition of ethanol. Biotechnol. Lett. 21: 965-968.
    CrossRef
  17. Nemec T, Jernejc K. 2002. Influence of Tween 80 on lipid metabolism of an Aspergillus niger strain. Appl. Biochem. Biotechnol. 101: 229-238.
    CrossRef
  18. Nyanhongo GS, Gomes J, Gubitz GM, Zvauya R, Read JS, Steiner W. 2002. Decolorization of textile dyes by laccases from a newly isolated strain of Trametes modesta. Water Res. 36: 1449-1456.
    CrossRef
  19. Nyanhongo G, Gomes J, Cubitz G, Zvauya R, Read J, Steiner W. 2002. Production of laccase by a newly isolated strain of Trametes modesta. Bioresour. Technol. 84: 259-263.
    CrossRef
  20. Palmieri G, Giardina P, Bianco C, Fontanella B, Sannia G. 2000. Copper induction of laccase isoenzymes in the ligninolytic fungus Pleurotus ostreatus. Appl. Environ. Microbiol. 66: 920-924.
    Pubmed CrossRef
  21. Paramjit KB, Daljit SA. 2009. Comparative production of ligninolytic enzymes by Phanerochaete chrysosporium and Polyporus sanguineus. Can. J. Microbiol. 55: 1397-1402.
    Pubmed CrossRef
  22. Patel H, Gupte A, Gupte S. 2009. Effect of different cultural conditions and inducers on production of laccase by a basidiomycete fungal isolate Pleurotus ostreatus HP-1 under solid state fermentation. Bioresources 4: 268-284.
  23. Pointing SB, Pelling AL, Smith GJD, Hyde KD, Reddy CA. 2005. Screening of basidiomycetes and xylariaceous fungi for lignin peroxidase and laccase gene-specific sequences. Mycol. Res. 109: 115-124.
    Pubmed CrossRef
  24. Pointing SB, Jones EBG, Vrijmoed LLP. 2000. Optimization of laccase production by Pycnoporus sanguineus in submerged liquid culture. Mycologia 92: 139-144.
    CrossRef
  25. Power T, Ortoneda M, Morrissey JP, Dobson ADW. 2006. Differential expression of genes involved in iron metabolism in Aspergillus fumigatus. Int. Microbiol. 9: 281-287.
    Pubmed
  26. Rodríguez A, Falcon MA, Carnicero A, Perestelo F, De la Fuente G, Trojanowski J. 1996. Laccase activities of Penicillium chrysogenum in relation to lignin degradation. Appl. Microbiol. Biotechnol. 45: 399–403.
    CrossRef
  27. Savoie JM, Mata G, Billette C. 1998. Extracellular laccase production during hyphal interactions between Trichoderma sp. and Shiitake, Lentinula edodes. Appl. Microbiol. Biotechnol. 49: 589-593.
    CrossRef
  28. Sethuraman A, Akin DE, Erriksson KE. 1999. Production of ligninolytic enzymes and synthetic lignin mineralization by the bird’s nest fungus Cyathus stercoreus. Appl. Mcirobiol. Biotechnol. 52: 689-697.
    Pubmed CrossRef
  29. Sharma KK, Kapoor M, Kuhad RC. 2005. In vivo enzymatic digestion, in vitro xylanase digestion, metabolic analogues, surfactants and polyethylene glycol ameliorate laccase production from Ganoderma sp. kk-02. Lett. Appl. Microbiol. 41: 24-31.
    Pubmed CrossRef
  30. Soden DM, Dobson ADW. 2001. Differential regulation of laccase gene expression in Pleurotus sajor-caju. Microbiology 147: 1755-1763.
    Pubmed
  31. Stajic M, Persky L, Friesem D, Hadar Y, Wasser SP, Nevo E, et al. 2006. Effect of different carbon and nitrogen sources on laccase and peroxidases production by selected Pleurotus species. Enzyme Microb. Technol. 38: 65-73.
    CrossRef
  32. Vasconcelos AFD, Barbosa AM, Dekker RFH, Scarmínio IS, Rezende MI. 2000. Optimization of laccase production by Botryosphaeria sp. in the presence of veratryl alcohol by the response-surface method. Process Biochem. 35: 1131-1138.
    CrossRef
  33. Velazquez-Cedeiio MA, Farnet AM, Ferre E, Savoie JM. 2004. Variations of lignocellulosic activities in dual cultures of Pleurotus ostreatus and Trichoderma longibrachiatum on unsterilized wheat straw. Mycologia 96: 712-719.
    CrossRef
  34. Warsinke A, Benkert A, Scheller FW. 2000. Electrochemical immunoassays. Fresen. J. Anal. Chem. 366: 622-634.
    Pubmed CrossRef
  35. Zhang SX, Xiao YZ, Wang YP. 2004. Immobilization of fungal laccase on nylon net and application of the immobilized enzyme. Microbiology 31: 85-88.



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