2018 ; 28(8):
|Author||Jose F. Garcia-Mazcorro, Romina Pedreschi, Boon Chew, Scot E Dowd, Jorge R Kawas, Giuliana D Noratto|
|Affiliation||Research and Development, MNA de México, San Nicolás de los Garza 66477, México|
|Title||Dietary Supplementation with Raspberry Extracts Modifies the Fecal Microbiota in Obese Diabetic db/db Mice|
J. Microbiol. Biotechnol.2018
|Abstract||Raspberries are polyphenol-rich fruits with the potential to reduce the severity of the clinical
signs associated with obesity, a phenomenon that may be related to changes in the gut
microbiota. The aim of this study was to investigate the effect of raspberry supplementation
on the fecal microbiota using an in vivo model of obesity. Obese diabetic db/db mice were
used in this study and assigned to two experimental groups (with and without raspberry
supplementation). Fecal samples were collected at the end of the supplementation period (8
weeks) and used for bacterial 16S rRNA gene profiling using a MiSeq instrument (Illumina).
QIIME 1.8 was used to analyze the 16S data. Raspberry supplementation was associated with
an increased abundance of Lachnospiraceae (p = 0.009), a very important group for gut health,
and decreased abundances of Lactobacillus, Odoribacter, and the fiber degrader S24-7 family as
well as unknown groups of Bacteroidales and Enterobacteriaceae (p < 0.05). These changes
were enough to clearly differentiate bacterial communities accordingly to treatment, based on
the analysis of UniFrac distance metrics. However, a predictive approach of functional profiles
showed no difference between the treatment groups. Fecal metabolomic analysis provided
critical information regarding the raspberry-supplemented group, whose relatively higher
phytosterol concentrations may be relevant for the host health, considering the proven health
benefits of these phytochemicals. Further studies are needed to investigate whether the
observed differences in microbial communities (e.g., Lachnospiraceae) or metabolites relate to
clinically significant differences that can prompt the use of raspberry extracts to help patients
|Keywords||Raspberry, polyphenols, obesity, gut microbiota|
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