2017 ; Vol.27-4: 856~867
|Author||Shimin Chang, Xingtian Cui, Mingzhang Guo, Yiling Tian, Wentao Xu, Kunlun Huang, Yuxing Zhang|
|Place of duty||College of Horticulture, Agricultural University of Hebei, Baoding 071001, P.R. China,College of Agriculture, Hebei University of Engineering, Handan 056038, P.R. China|
|Title||Insoluble Dietary Fiber from Pear Pomace Can Prevent High-Fat Diet-Induced Obesity in Rats Mainly by Improving the Structure of the Gut Microbiota|
J. Microbiol. Biotechnol.2017 ;
|Abstract||Supplement of dietary fibers (DF) is regarded as one of the most effective way to prevent and
relieve chronic diseases caused by long-term intake of a high-fat diet in the current society.
The health benefits of soluble dietary fibers (SDF) have been widely researched and applied,
whereas the insoluble dietary fibers (IDF), which represent a higher proportion in plant food,
were mistakenly thought to have effects only in fecal bulking. In this article, we proved the
anti-obesity and glucose homeostasis improvement effects of IDF from pear pomace at first,
and then the mechanisms responsible for these effects were analyzed. The preliminary study
by real-time PCR and ELISA showed that this kind of IDF caused more changes in the gut
microbiota compared with in satiety hormone or in hepatic metabolism. Further analysis of
the gut microbiota by high-throughput amplicon sequencing showed IDF from pear pomace
obviously improved the structure of the gut microbiota. Specifically, it promoted the growth
of Bacteroidetes and inhibited the growth of Firmicutes. These results are coincident with
previous hypothesis that the ratio of Bacteroidetes/Firmicutes is negatively related with
obesity. In conclusion, our results demonstrated IDF from pear pomace could prevent high-fat
diet-induced obesity in rats mainly by improving the structure of the gut microbiota.|
|Key_word||Insoluble dietary fiber, anti-obesity, gut microbiota, amplicon sequencing|
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